Importantly, the ectopic expression approach allowed us to test also a C-terminal truncation resulting in deletion of the entire BID domain and positively charged tail sequence (3xFLAG-BepCBhe(FIC-OB), Fig 3A) that could not be tested in the infection assay as deletion of this bipartite secretion signal abolishes VirB/VirD4-dependent protein translocation [8]. This evidence concerns the gene BID and infection.