Synovial fluid mononuclear cells (SFMC) from HLA-DRB1*04:01 RA patients (n = 7) were stimulated with the 5 immunogenic cit-TNC peptides or their corresponding arg-TNC peptides, and cytokine secretion (IFN-γ/IL-17/IL-10) was then assessed using a FluoroSpot assay. This evidence concerns the gene HLA-DRB1 and rheumatoid arthritis.