Therefore, according to the effects of fucoidan in the up-regulation of Bax, cleaved caspase-3, and cleaved PARP, down-regulation of Bcl-2, and alteration of ΔΨm, it could be implied that fucoidan has potential in apoptotic induction via an intrinsic pathway on CL-6 cholangiocarcinoma cell. This evidence concerns the gene BCL2 and cholangiocarcinoma.