The expression matrix of five important EMT markers including TWIST1, ZEB1, VIM, ZEB2, and CDH2 in CRC from TCGA was used to perform the hierarchical clustering analysis to divide the TCGA CRC samples into two groups: epithelial-like phenotype (green) and mesenchymal-like phenotype (red) (Figure 3(a)). Here, TWIST1 is linked to colorectal carcinoma.