Several animal models of dysregulated signaling (growth factors and transcription factors) result in colobomas and exhibit a failure of BM breaching, as determined by intact BM generally shown by laminin staining (Macdonald et al., 1997; Barbieri et al., 1999; Sehgal et al., 2008; Chen et al., 2012; Cai et al., 2013). Here, LAMB2 is linked to coloboma.