We synthesized agomir and pcDNA3 to infect neurons to achieve stable overexpression of miR-410 and HDAC1. At 24-h after infection, RT-qPCR and immunoblotting (Figures 5B,F,G) displayed downregulation of miR-410 and upregulation of HDAC1 in OGD-exposed neurons compared to untreated neurons. This evidence concerns the gene HDAC1 and infection.