CASP3 and cholangiocarcinoma: Furthermore, anti-apoptotic proteins including Bcl-XL and Mcl-1 were significantly decreased (* P < 0.05), whereas Bax pro-apoptotic protein and cleaved caspase-3 were increased noticeably in reversine treatment for 48 and 72 h in both CCA cell lines (Figs. 2D–2F).