The validation in Oncomine and GEPIA2 confirms that the mRNA levels of TOP2A, CCNB2, PRC1, RACGAP1, AURKA, CDKN3, NUSAP1, CDCA5, ASPM, and NCAPG were significantly overexpressed in the HCC tissues, and at this validation, the p < 0.0001 and p < 0.01 were set in Oncomine and GEPIA2, respectively to more accurately identify the expression pattern of hub genes between HCC and normal tissues. The gene discussed is NUSAP1; the disease is hepatocellular carcinoma.