In addition, our analyses revealed that diet-induced FLHS resulted in genome-wide epigenetic alterations and enrichment of TF binding motifs in H3K27ac regions, including the binding motifs for ERG, FOXA1, GABPA, FLI1, ETV1, PHA-4, ELF1, FOXO1, ETS1, and ETV2, which are factors reported to be involved in immune system processes and metabolic homeostasis in FLHS chickens, in accordance with the results of the functional enrichment analysis of candidate differentially expressed genes. This evidence concerns the gene ELF1 and Floating-Harbor syndrome.