Raman spectral analysis showed that introducing the IDH1 mutation into a U251 glioblastoma cell line (U251R132H/C) increased lipid heterogeneity, as measured by the following parameters: (1) the lipid unsaturation parameter (the double bond contents, LSU) (Fig. 1c, i, j Supplementary Fig. 2d–f), (2) the trans/cis parameter (stereoisomers of fatty acids, TCP) (Fig. 1d, Supplementary Fig. 2g–i), (3) sphingomyelin (Fig. 1f, h, Supplementary Fig. 1e–g) and cholesterol levels (CL, Fig. 1e, Supplementary Fig. 2a–c). This evidence concerns the gene IDH1 and glioblastoma.