We identified the top 10 variables enriched in BC N+ as, respectively carrying 9% (Vδ2+ γδ TEMRA), 7% (Vδ2+ γδ CD57+), 4.5% (NK LAG3+, Vδ2+ γδ TIGIT+), 4.1% (Vδ2+ γδ TIM3+), 3.6% (αβCD4+ conventional TIGIT+), 3.4% (Vδ2+ γδ KIR2DL1/DS1+), 3% (Vδ2+ γδ ICOS+) and 2.7% (Vδ2+ γδ CD56+ αβCD4+ conventional TIGIT+) of contribution to the discrimination of groups (Figure 2B, waterfall plots, dark red arrow enriched in BC N+). The gene discussed is ICOS; the disease is breast cancer.