Using a second available dataset from patients with osteosarcoma, which were selected from cases with poor outcomes because of relapsed or metastatic disease, high PHGDH expression by RNA sequencing (RNA-seq) was also correlated with poor disease-free survival (DFS; p = 0.031; Figures S1A and patient characteristics presented in S1B). Here, PHGDH is linked to metastatic neoplasm.