HMGB1 and ischemia: There was no significant difference in plasma cTnT at 33°C and 36°C TTM (P = 0.999), indicating that both target core temperatures for TTM equivalently reduced myocardial damage (Fig 5D) Next, we performed an ELISA to measure HMGB1 levels in serum samples obtained at 4 h after the onset of ischemia.