As astrocyte dysfunction is associated with dementia and brain ageing but is further important area [12], we also compared the expression of GFAP (a marker that reflects astrocyte hypertrophy) and EAAT-2 (a marker that reflects altered glial function) in the studied astrocytes because double labelling shows that GFAP and EAAT2 do not entirely overlap [31]. This evidence concerns the gene SLC1A2 and dementia.