According to the results of the EdU proliferation assay and the colony formation assay, decreased expression of SPRR1B inhibited lung adenocarcinoma proliferation, induced G2/M‐phase arrest, and promoted apoptosis, which differs from the reported enhanced entry of cells into the G0 phase discussed by Tesfaigzi et al.31Simultaneously, SPRR1B knockdown inhibited lung cancer cell migration and invasion, which indicates the potential role of SPRR1B in tumor invasion and metastasis, although further study is also needed. This evidence concerns the gene SPRR1B and lung adenocarcinoma.