Our results showed that the co-delivery of 5-FU and miR-34a(m) not only substantially enhanced the anti-CRC activity of 5-FU by silencing sirt1 expression but also suppressed CRC cell migration by targeting CD44, suggesting that co-delivery of 5-FU and miR-34a(m) could achieve synergistic effects on tumor suppression with undetectable side effects in vitro and in vivo. The gene discussed is SIRT1; the disease is neoplasm.