p-STAT3, NEK9 and GP130 are simultaneously upregulated in GC metastases and correlate with poor prognosis in GC patients. To evaluate the clinical significance of p-STAT3, NEK9 and GP130 in GC patients, the expression of p-STAT3, NEK9 and GP130 was analysed in 2 sets of GC tissue microarrays via IHC. This evidence concerns the gene NEK9 and gastric cancer.