To clarify whether the downregulation of PPARγ in ob/ob macrophages observed in Figure 7C resulted from obesity itself or LPS + nigericin treatment, we examined basal, untreated PPARγ levels from control and ob/ob macrophages, and found that basal, untreated PPARγ levels remained similar between the two groups (Figure S6D-E). This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.