Moreover, mRNA expression levels of Sqstm1, Optn, LC3b, and Becn1 were higher, protein level of Aβ was lower, protein level of BECN1, SQSTM1, OPTN, ratio of LC3B-II:I were higher in brain tissues of AD mice treated with miR-331-3p antagomir or miR-9-5p antagomir, or both together than that in control AD mice (Figure 6A-E). The gene discussed is SQSTM1; the disease is Alzheimer disease.