PCBP2 and neoplasm: After a 2 h incubation with Hoechst 33258, a 2-fold higher fluorescence intensity of Hoechst 33258 was observed 150 μm from the periphery in tumor spheroids treated with the CCP/PCBP2 siRNA nanocomplex than in spheroids treated with the CCP/scrambled siRNA nanocomplex, and a 1.6-fold stronger fluorescence signal was observed after a 4 h incubation (Figure 6C-D).