The nuclear sub-localization of DNMT1 was also investigated and the results showed PGC7 disturbed the localization of DNMT1 on the nuclear speckles (SC35 staining) in both cell lines (Fig. 6d), indicating UHRF1–DNMT1 function was disturbed by PGC7 in HCC cells. This evidence concerns the gene DNMT1 and hepatocellular carcinoma.