Cell viability and flow cytometry analysis showed that the frequencies of apoptotic cells were significantly decreased in PGC7-transfected MIHA and 97H cells (Fig. 2c, d), as well as HCC patient-derived organoids (PDOs) (Fig. 2e, f), indicating PGC7 confers chemoresistance property to HCC cells. This evidence concerns the gene DPPA3 and hepatocellular carcinoma.