Bisulfite Sanger sequencing was performed for the promoter of ITGA7, which evaded from ENCODE RRBS and XmaI-RRBS, to confirm its nonmethylated status in normal and tumor samples; and for one of the promoters (NID2) that demonstrated differential methylation by MSRE-PCR, to validate nonmethylated status in normal samples and abnormal methylation in cancer. The gene discussed is ITGA7; the disease is neoplasm.