EIF2A and Zika virus infectious disease: ZIKV infection of placental trophoblast showed increased levels of phosphorylated eIF2α (P-eIF2α) after 24 h of infection in JEG-3 and HTR-8 cells, whereas activation of eIF2α was observed after 16 h of infection in JAR cells and the levels of total eIF2α remain unaltered (Fig. 5a).