EIF2AK4 and infection: To address the function of TgIF2K-B during infection and assess which stress condition(s) activates TgIF2K-B, we treated WT and Δtgif2k-b parasites with the following stress agents: thapsigargin (TG), an ER stress inducer that leads to activation of TgIF2K-A (10), halofuginone (HF), which inhibits the aminoacylation of tRNAPro and is also a potent inducer of the eIF2α kinase GCN2, and sodium arsenite (Ars), which triggers general oxidative stress.