IFNG and neoplasm: Ex vivo splenocyte analysis demonstrated that at day 8 (4 days following the last of three treatments as shown in online supplemental figure S7B), CAL-101/VVL-21 enhanced splenocyte IFN-γ expression in response to mitomycin C-treated DT6606 tumor cells and inclusion of α-PD1 into the therapeutic regime improved IFN-γ induction (figure 5C), with the difference being more pronounced by day 10 (6 days following the last injection) (figure 5D).