The lower adiposity in HFHC diet-fed LRP1 NPxY mutant mice was reflected by their lower leptin expression levels in adipose tissues, leading to lower plasma leptin levels in HFHC diet-fed LRP1 NPxY mutant mice than in wild-type mice on the same diet or in mice fed the HF diet without cholesterol supplementation (Fig. 1C, D). This evidence concerns the gene LEP and hydrops fetalis.