In the current study, we demonstrated that SUFU accumulation inactivated the hedgehog signaling upon ROC1 knockdown, while blockage of SUFU expression restored the hedgehog signaling suppression triggered by the ROC1 knockdown, indicating that the ROC1-regulated hedgehog signaling in bladder cancer was dependent on SUFU degradation. Here, RBX1 is linked to urinary bladder carcinoma.