The results showed that these mRNAs have potential roles in cell cycle, various cancers, miRNA in cancer, DNA-binding transcriptional activator activity, TGF-β signalling pathway, transcription factor complex, RNA polymerase II specificity, etc. Further, univariate Cox regression and multivariate Cox regression analyses were used to construct a Cox proportional hazards regression model with 13 key genes: CDK6, FAM84B, FBLIM1, FJX1, GNB5, HOXA3, MLEC, PDXK, SOX11, SPRY4, TBPL1, TRIB2, and WEE1. We calculated the risk score of patients, and verified that the prognostic model is highly accurate. This evidence concerns the gene GNB5 and cancer.