ASPG and sarcoma: These differences were accompanied by lower aspartate, glutamate and glutamine content in asparaginase-treated cells (W-A-N0, W-A-N100) compared to control cells (W-0-N0) (Figure 5g) and reminiscent of the changes observed in shASNS sarcoma cells cultured in asparagine-free medium (Figure 3 and Figure 4).