ASPG and sarcoma: Asparaginase-treated sarcoma cells supplemented with exogenous asparagine (W-A-N100, marked in green) clustered with asparaginase-treated cells cultured in asparagine-free medium (W-A-N0, marked in red), but there were marked differences between asparaginase-treated (W-A-N0, W-A-N100) and control sarcoma cells (W-0-N0, W-0-N100, marked in shades of blue) (Figure 5c, Table S5).