To investigate the effects of asparagine availability on the metabolome of KRAS-driven mouse sarcoma cells, asparagine availability was modified by exposing shASNS sarcoma cells (A), shLuc control sarcoma cells (C) and wild-type sarcoma cells (W) to asparagine-free medium or medium containing near-physiological asparagine concentrations (5 mg/L) or supraphysiological asparagine concentrations (100 mg/L) for 2 days (Figure 3a). This evidence concerns the gene KRAS and sarcoma.