Figure 5B represents staining of VASA (pre-meiotic), BOULE (meiotic) and ACROSIN (meiotic/post meiotic) from cells before culture (BC; to identify type of spermatogenic cells before culture) and from developed cells in MCS in the absence or the presence of PEDF in different concentration (1–1000 ng/mL). The gene discussed is SERPINF1; the disease is breast cancer.