The expression of EE-tagged Pank1β was analyzed by anti-EE Western Blot (WB) in HEK293 cells transfected with pLex vector alone (Figure 1B, Lane 1), in the HEK293/Pank1β stable cell line generated by infection with the pLex/Pank1β lentivirus construct followed by puromycin selection (Figure 1B, Lane 2), and in HEK293 cells transiently transfected with the pLex/Pank1β lentivirus construct (Figure 1B, Lane 3). The gene discussed is PANK1; the disease is infection.