These findings indicated that MT-RNR1 709A was significantly associated with distant metastasis-free survival in the subgroups of male sex, cirrhosis positivity, HBV-related HCC, AFP ≥ 400 ng/mL, AST ≥ 31 IU/L, PT ≥ 12 s, tumor number > 1, tumor size ≥ 5 cm, histology grade 3-4, and macrovascular invasion positivity. Here, F2 is linked to hepatocellular carcinoma.