Such experiments would require (1) the inactivation of IL2Rα and its downstream targets in tumor cells upon co-culture with Tregs; (2) a larger approach such as RNA sequencing or proteomics analysis on IL2Rα+ tumor cells to fully disclose the signaling pathways activated by IL2Rα expression; and (3) a quantitative demonstration that spheroid growth was due to enhanced tumor cell proliferation. The gene discussed is IL2RA; the disease is neoplasm.