It has been shown that in severely obese individuals with steatosis, the hepatic content of diacylglycerol (DAG) correlated positively with liver TG content and negatively with insulin-mediated suppression of glucose production [47], and it was shown in obese individuals that lipid-droplet associated DAG in liver samples correlated with HOMA-IR, and with protein kinase C ε (PKCε) activation, the main PKC isoform in human liver, which impairs insulin signaling [48]. This evidence concerns the gene INS and steatosis.