In separate wells, along with the differentiation cocktail, several methods to induce insulin resistance were undertaken including: 10% high serum (HS), 100 nM and 200 nM hyperinsulinemia (HI100, HI200), 1 μM dexamethasone (Dex), 2.5 nM TNFα, and a combination of hyperinsulinemia and TNFα. The gene discussed is TNF; the disease is hyperinsulinism.