To explore the possibility that CD6-CD318 could be a potential target for lymphocyte checkpoint inhibition, we used an IncuCyte imaging device to image cocultures of cancer cell lines and PBMCs or purified lymphocyte subsets, with or without anti-CD6 (UMCD6) or anti-CD318 (3a11) mAbs. This evidence concerns the gene CDCP1 and cancer.