Down-regulated gene sets included those associated with extracellular matrix interactions, chaperone function, calnexin/calreticulin cycle, N-glycan trimming and peptide chain elongation (Fig. 2A), while gene sets upregulated in response to hyperglycaemia included cholesterol biosynthesis, complement cascade and fibrin clotting cascade (Fig. 2B–D). This evidence concerns the gene CALR and Hyperglycemia.