To test if Cav1 deficiency could rescue CCM phenotypes in the mouse CCM models, Pdcd10BECKO:Cav1−/− (DKO) mice were obtained by mating with Cav1−/− mice with Mfsd2a-CreERT2:Pdcd10fl/fl mice, followed by feeding pups with tamoxifen at P1 to P3 for induction of severe lesions. This evidence concerns the gene MFSD2A and cerebral cavernous malformation.