TNK2 and cancer: In addition, we retrieved RNA-seq data for 188 lung cancer cell lines from the Cancer Cell Line Encyclopedia (CCLE) database and dichotomized the cancer cell lines by the average expression levels of the ACK1. Gene set enrichment analysis (GSEA) performed between the ACK1high and ACK1low groups confirmed the enrichment of ACK1-regulated genes in the MAPK, Wnt, NSCLC, and axon guidance pathways (Figure 5A).