Decreased luciferase activity was found in constructs for three genes (SMS, PAFAH1B2, and PDK3) in HEK293T or MCF-7 cells (Fig. 5c) and their binding sites with miR-3613-3p are shown in Supplementary Fig. 11A. More importantly, the highly amplified transcripts of SMS and PDK3 were observed in breast cancer samples with miR-3613 deletion compared to the non-deletion group from TCGA breast cancer cohort (Fig. 5d). The gene discussed is SMS; the disease is breast carcinoma.