S. nigrum may exert anti-tumor effects in BC patients mainly by regulating the BC pathway (hsa05219), MAPK signaling pathway (hsa04010), HIF-1 signaling pathway (hsa04066), TNF signaling pathway (hsa04668), apoptosis signaling pathway (hsa04210), PI3K-Akt signaling pathway (hsa04151), p53 signaling pathway (hsa04115), and NF-kappa B signaling pathway (hsa04064). This evidence concerns the gene TNF and neoplasm.