In our experimental setup, in human osteosarcoma and adenocarcinoma cells, addition of GR caused an increase in RANKL mRNA level which is similar to previously reported results obtained on dexamethasone treated human primary osteoblast [44] and mouse bone marrow stromal cells treated with dexamethasone and vitamin D3 [41,47]. The gene discussed is TNFSF11; the disease is osteosarcoma.