Li et al. at research assessing the value of serum NGAL in identifying early acute kidney injury induced by a high dose of methotrexate (HDMTX) concluded that the combination of serum creatinine ratio and serum NGAL after 24 h of cytostatic infusion had higher value for early diagnosis of HDMTX associated with AKI compared with serum creatinine only [20]. The gene discussed is LCN2; the disease is acute kidney injury.