In our NAFLD mouse model, the RNAseq data displayed a dramatic downregulation of numerous genes in primary bile acid biosynthesis (Figure S8A), including the two rate-limiting enzymes for bile acid synthesis, Cyp7a1(Cholesterol 7 alpha-hydroxylase) and Cyp27a1. Here, CYP27A1 is linked to metabolic dysfunction-associated steatotic liver disease.