Our results showed that more than 90% of cases diagnosed as primary adenocarcinoma of the gallbladder were positive for CK7 (97.6%), CK19 (98.2%), CKLMW (100%), CKHMW (91.6%), or MUC1 (97.1%) expression, or were negative for vimentin (96.1%) or MUC2 (96.9%). Here, VIM is linked to adenocarcinoma.