To define the overall contributions of TRPC channels in brain damage associated with cerebral ischemia, we first compared neurological scores and brain infarction volumes of wild type and quadruple TRPC1, C4, C5, C6 knockout (QuadKO) mice subject to 40 min of MCAO using the standard intraluminal suture (thread) method, followed by 24 h reperfusion. Here, TRPC1 is linked to brain ischemia.