AKT1 and neoplasm: Levels of pAKT were found increased in MB231-GPS2KO both by WB of cellular extracts and in tumor samples taken from orthotopic xenograft models, in agreement with the enrichment for AKT substrates observed by phospho-proteomic profiling and with previous findings from our lab showing that aberrant upregulation of AKT ubiquitination promoted sustained AKT signaling in GPS2 null adipocytes (Cederquist et al., 2017).