To further characterize the effect of GPS2 depletion in MDA-MB-231 cells and investigate the underlying molecular mechanisms by which GPS2 acts as a tumor suppressor, we performed proteomic and phosphoproteomic profiling of five independent replicate samples of MDA-MB-231 WT and GPS2KO cells by LC-MS/MS, using isobaric tandem mass tag (TMT) labeling for relative quantification. This evidence concerns the gene GPS2 and neoplasm.