In this study, we systematically analyzed the expression, molecular cascade, and function of the endosomal RNA sensor TLR3; cytosolic RNA sensors RIG-I, MDA5, and LGP2; and cytosolic DNA sensors cGAS and STING in 22 human epithelial cancer cell lines to obtain useful insights into targeting nucleic acid receptors for cancer immunotherapy. This evidence concerns the gene IFIH1 and cancer.