To investigate their roles in human epithelial cancer cells, we collected 22 human epithelial cancer cell lines derived from nine cancer types, including breast cancer, cervical cancer, colorectal cancer, gastric cancer, glioma, human hepatocellular cancer, lung cancer, human ovarian cancer, and human pancreatic cancer (Supplementary Table 1) and detected the major molecules in the TLR3–TRIF, RLR–MAVS, and cGAS–STING pathways in these cells, including TLR3, TRIF, RIG-I, MDA5, LGP2, MAVS, cGAS, and STING. Here, TLR3 is linked to central nervous system cancer.