In fact, the enzymatic assays on the lysates of the treated cells of different BC subtypes showed the strong inhibition of TrxR seleno-enzyme and, surprisingly, of another selected enzyme such as DHFR in an extent not so much dissimilar, despite the fact that inhibition tests in vitro on the activity of these enzymes afforded to values of Ki different up to an order of 103 (Galassi et al., 2012, 2015). This evidence concerns the gene DHFR and breast cancer.