To demonstrate that macitentan exerts a direct anti-angiogenic activity in MM in vivo, we subsequently set-up a chick embrio CAM assay using RPMI-8226 cells, which express basal levels of VEGF-A, IL-8 and ADM, other than ET-1 (Figures 4B, 5B, C). This evidence concerns the gene ADM and Miyoshi myopathy.